Practical Methods for Biocatalysis andBiotransformations by John Whittall, Peter Sutton
English | 2010 | ISBN: 0470519274 , 0470748591 | 336 pages | PDF | 5,1 MB
Biocatalysts are increasingly used by chemistsengaged in fine chemical synthesis within both industry and academia. Today,there exists a huge choice of high-tech enzymes and whole cell biocatalysts,which add enormously to the repertoire of synthetic possibilities.
Practical Methods for Biocatalysis andBiotransformations is a “how-to” guide focussing on commercially availableenzymes and strains of microorganisms that are readily obtained from culturecollections. The source of starting materials and reagents, hints, tips andsafety advice (where appropriate) are given to ensure, as far as possible, thatthe procedures are reproducible. Comparisons to alternative methodology aregiven and relevant references to the primary literature are cited. Contentsinclude:
•Introduction to the application of biocatalystsin the pharma industry
•introduction to molecular biological techniquesfor biocatalyst development
•Lipase and protease methods including enzymesupports and dynamic kinetic resolutions
•Nitrile hydrolases and hydratases
•Alcohol dehydrogenases and co-factor recycling
•Epoxide hydrolases
•Amino acid synthesis
•Whole cell processes
•Other biocatalysts, includingmonoamineoxidases, aminotransterases, and lyases
Practical Methods for Biocatalysis andBiotransformations is an essential text offering validated biocatalyst methodsfor researchers and postgraduate students in industry and academia.

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